Hydroxyl Radical Generation by Mammalian Catalase: A Few Experimental Evidences
نویسندگان
چکیده
منابع مشابه
Hydroxyl radical generation theory: a possible explanation of unexplained actions of mammalian catalase.
Catalase is well known antioxidant enzyme which catalyses the dissociation of hydrogen peroxide directly into H(2)O and O(2). Mammalian catalase has been considered as 'a venerable enzyme with new mysteries'. Some aspects of its mechanism of action are mystifying and many of new findings are still unexplained. To fill up the gap we propose the 'Hydroxyl Radical Generation Theory (HRGT)' with po...
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The photogeneration of hydroxyl radicals (OH(*)) in photosystem II (PSII) membranes was studied using EPR spin-trapping spectroscopy. Two kinetically distinguishable phases in the formation of the spin trap-hydroxyl (POBN-OH) adduct EPR signal were observed: the first phase (t(1/2) = 7.5 min) and the second phase (t(1/2) = 30 min). The generation of OH(*) was found to be suppressed in the absen...
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Xanthine oxidase has been hypothesized to be an important source of biological free radical generation. The enzyme generates the superoxide radical, .O2- and has been widely applied as a .O2- generating system; however, the enzyme may also generate other forms of reduced oxygen. We have applied electron paramagnetic resonance (EPR) spectroscopy using the spin trap 5,5'-dimethyl-1-pyrroline-N-ox...
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The hydroxyl radicals are known to be a biologically active oxygen species. A remarkable enhancement of hydroxyl radical generation was observed in the mitochondria obtained from ischemic myocardium. The hydroxyl radicals are the primary reactive species leading to cellular damages, such as membrane damages, DNA damages, enzyme inactivation, protein denaturation and so forth. The origin of the ...
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ژورنال
عنوان ژورنال: Biochemistry & Pharmacology: Open Access
سال: 2012
ISSN: 2167-0501
DOI: 10.4172/2167-0501.1000103